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Restriction enzyme: History & Applications

A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types, which…

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Restriction enzyme topic overview

The analysis highlights History and Applications as prominent areas in the source structure around Restriction enzyme.

Related topics
131
Source areas
7
Connected nodes
138
Extracted relationships
201
Concept neighborhoods
31
Bridge connections
138

What this topic covers Research coverage

Source areas are shown by the number of related topics found in each part of the analysis. Use smaller areas too: they can reveal specialized angles and content gaps.

Types · 43 topics
Applications · 37 topics
History · 21 topics
Overview · 16 topics
Recognition site · 8 topics
Nomenclature · 4 topics
Origins · 2 topics

Smaller areas are not necessarily less important. They contain fewer connections in this analysis and can be useful for finding specialized angles or coverage gaps.

Key facts & relationships

High-confidence facts extracted from structured source data. Use them as anchors for further research.

AlphaFold
IPR011335
Available protein structures:
IPR011335
InterPro
IPR011335
PDB
IPR011335
Pfam clan
CL0236
SCOP2
1wte / SCOPe / SUPFAM

Explore all related topics Closing gaps

Browse the complete topic structure, not only the most central items. Less prominent entities and concepts can reveal missing angles, specialized context and useful research gaps. Each item opens a new analysis centered on that subject.

Overview

History

Origins

Recognition site

Types

Nomenclature

Applications

Advanced semantic analysis

Deeper signals for content research, entity SEO and topical coverage. The plain-language headings explain what each technical view is useful for.

How Restriction enzyme connects Entity context

The extracted context around Restriction enzyme shows recurring relationship patterns in the source. For example, Restriction enzyme → AdoMet, ATP, BcgI, BplI, Bpu10I, BslI, DNA, DpnI, Eco57I, FokI, Golden Gate, II, IIE, IIF, In, It, Mg2, NaeI, NgoMIV, One Another extracted example is Restriction enzyme → Arber, Daniel Nathans, DNA, Escherichia, For, Giuseppe Bertani, Haemophilus, Hamilton, HindII, If, II, In, It, Jean Weigle, Kathleen Danna, Kent Wilcox, Later, Matthew Meselson, Medicine, Meselson. Use these groups to spot repeated connection types before inspecting the individual relationships.

Restriction enzyme

Top relations

related to Type II · 34
Restriction enzyme → AdoMet, ATP, BcgI, BplI, Bpu10I, BslI, DNA, DpnI, Eco57I, FokI, Golden Gate, II, IIE, IIF, In, It, Mg2, NaeI, NgoMIV, One
related to history · 29
Restriction enzyme → Arber, Daniel Nathans, DNA, Escherichia, For, Giuseppe Bertani, Haemophilus, Hamilton, HindII, If, II, In, It, Jean Weigle, Kathleen Danna, Kent Wilcox, Later, Matthew Meselson, Medicine, Meselson
related to External links · 28
Restriction enzyme → Archived, DNA Restriction Enzymes, Firman, Goodsell DS, Macelis, Medicine Medical Subject Headings, MeSH, Molecular Scissors, Molecule, Month, National Library, Portsmouth, Posfai, RCSB Protein Data Bank, REBASE, Restriction Endonucleases, Restriction Enzyme Database, Restriction Enzymes, Restriction-Modification, Retrieved
related to Type III · 19
Restriction enzyme → AdoMet, ATP, DNA, EcoP15, FXGXG, IV, Mod, N-6, N6, P08763, P08764, PP, Res, S/D/N, The Mod, These, They, Type III, Y/F
related to Types · 18
Restriction enzyme → All, ATP, DNA, DNAType, EC2, EC3, II, III, IV, Naturally, RNAs, S-adenosyl-L-methionine, They, Type, Type II, Type III, Type IV, Types
related to Artificial restriction enzymes · 14
Restriction enzyme → Artificial, Clustered, CRISPR, CRISPR-Cas9, DNA, DNA-binding, FokI, For, IIS, In, Other, Such, TAL, Zinc
related to Type l · 14
Restriction enzyme → AdoMet, ATP, Cleavage, DNA, DNA-binding, HsdM, HsdR, HsdS, K-12, Mg2, S-Adenosyl, The, These, Type
related to Recognition site · 10
Restriction enzyme → CATATG, DNA, EcoRI, GTAATG, GTATAC, In, Inverted, Many, Restriction, The
has application · 7
Restriction enzyme → DNA, For, Isolated, MCS, They, This, To
related to Type V · 7
Restriction enzyme → CRISPRs, DNA, RNA, RNAs, The, They, Type

Important terminology

Use these terms to understand the vocabulary surrounding the topic, not as a checklist for keyword stuffing.

Important terminology

dna restriction enzymes type recognition enzyme site sites sequence cleavage two used cut one cleave endonucleases sequences also gene specific

Restriction enzyme relationships Subject–Predicate–Object triples

TTTA extracted 201 structured relationships around Restriction enzyme. Examples in this analysis include Restriction enzyme → AlphaFold → IPR011335 and Restriction enzyme → Pfam clan → CL0236. The table shows each extracted connection, where it came from and its confidence.

SubjectPredicateObjectConfidenceSrc
Restriction enzymeAlphaFoldIPR0113351.00infobox
Restriction enzymeAvailable protein structures:IPR0113351.00infobox
Restriction enzymeInterProIPR0113351.00infobox
Restriction enzymePDBIPR0113351.00infobox
Restriction enzymePfam clanCL02361.00infobox
Restriction enzymeSCOP21wte / SCOPe / SUPFAM1.00infobox
Restriction enzymeSymbolRestrct_endonuc-II-like1.00infobox
human insulin used by diabetic patientsinstance ofallowing the large scale production of proteins0.80text
Golden Gate cloninginstance ofthis characteristic is widely used to perform in-vitro cloning techniques0.80text
Restriction enzymehas applicationIsolated0.60section
Restriction enzymehas applicationDNA0.60section
Restriction enzymehas applicationThey0.60section

Related concept clusters Concept neighborhoods

The concept neighborhoods around Restriction enzyme bring nearby vocabulary together. In this analysis, examples include Enzymes, Type and Restriction. Use the clusters to find adjacent concepts and terminology that may deserve separate research.

  • Restriction enzyme
    • Enzymes
    • Type
    • Restriction
    • Dna
    • Used
    • Recognition
    • Sequence
    • Digestion
    • Cleavage
    • Endonucleases
    • Site
    • Two
  • restriction enzyme
    • Enzymes
    • Type
    • Cleavage
    • Ecori
    • Ii
    • Restriction
    • Dna
    • Used
    • Recognition
    • Sequence
    • Digestion
    • Endonucleases
  • enzyme
    • Cleavage
    • Ecori
    • Ii
    • Restriction
    • Dna
    • Sites
    • Site
    • One
    • Recognition
    • Type
    • Sequence
    • Endonuclease
  • dna
    • Restriction
    • Enzymes
    • Cut
    • Enzyme
    • Used
    • Cleavage
    • Recognition
    • Sequence
    • Cleave
    • Sites
    • Site
    • Type
  • restriction sites
    • Enzymes
    • Type
    • Cleavage
    • Two
    • Used
    • Recognition
    • Sequence
    • Digestion
    • Require
    • Site
    • Endonucleases
    • One
  • dna double helix
    • Restriction
    • Enzymes
    • Cut
    • Enzyme
    • Used
    • Cleavage
    • Recognition
    • Sequence
    • Cleave
    • Sites
    • Site
    • Type
  • restriction digestion
    • Enzymes
    • Require
    • Type
    • Modification
    • Recognition
    • Atp
    • Used
    • Sequence
    • Digestion
    • Restriction
    • Cleavage
    • Endonucleases
  • restriction modification system
    • Enzymes
    • Type
    • System
    • Used
    • Recognition
    • Sequence
    • Digestion
    • Specific
    • Cleavage
    • Endonucleases
    • Site
    • Two

Connections between topic areas Semantic bridges

For Restriction enzyme, one of the stronger structural bridges in this analysis connects Restriction enzyme with Types. Bridges highlight paths between different parts of the map and can reveal research angles that are easy to miss in a flat list.

Min side: 3
Restriction enzymeTypes · splits 95 ⟂ 44
Restriction enzymeApplications · splits 101 ⟂ 38
Restriction enzymeHistory · splits 117 ⟂ 22
Restriction enzymeOverview · splits 122 ⟂ 17
Restriction enzymeRecognition site · splits 130 ⟂ 9
Restriction enzymeNomenclature · splits 134 ⟂ 5
Restriction enzymeOrigins · splits 136 ⟂ 3

Map overview Semantic statistics

Restriction enzyme

Nodes139
Edges138
Triples201
Avg. degree1.99
Density0.014388
Components1

Source & methodology

TTTA analyzes the structure around Restriction enzyme to surface related topics, entities, relationships, concept neighborhoods and bridge connections. Use the map to explore areas such as History & Applications, including less central topics that may reveal useful research gaps. Automatically extracted connections are research leads rather than rewritten encyclopedia content.

Source: Wikipedia — Restriction enzyme · EN edition · Analysis: TopicsToTalkAbout

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