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In molecular biology, a restriction digest is a procedure used to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation, though this term is used for other procedures as well. In a restriction digest, DNA molecules are cleaved at specific regions of 4-12 nucleotides in length (restriction sites) by use of restriction…
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dna restriction enzymes gel digest used electrophoresis fragment site agarose sequences size specific also enzyme end may fragments nucleotides sequence
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| Restriction digest | is a | procedure used to prepare DNA for analysis or other processing | 0.90 | text |
| agarose gel electrophoresis | instance of | making it more suitable for analytical techniques | 0.80 | text |
| and chromatography | instance of | making it more suitable for analytical techniques | 0.80 | text |
| Restriction digest | related to Possible uses | Restriction | 0.60 | section |
| Restriction digest | related to Possible uses | DNA | 0.60 | section |
| Restriction digest | related to Possible uses | The | 0.60 | section |
| Restriction digest | related to Restriction digest by gel electrophoresis | In | 0.60 | section |
| Restriction digest | related to Restriction digest by gel electrophoresis | DNA | 0.60 | section |
| Restriction digest | related to Restriction digest by gel electrophoresis | Digestion | 0.60 | section |
| Restriction digest | related to Restriction digest by gel electrophoresis | The | 0.60 | section |
| Restriction digest | related to Restriction digest by gel electrophoresis | To | 0.60 | section |
| Restriction digest | related to Restriction site | DNA | 0.60 | section |
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