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RNA-Seq (short for RNA sequencing) is a next-generation sequencing (NGS) technique used to quantify and identify RNA molecules in a biological sample, providing a snapshot of the transcriptome at a specific time. It enables transcriptome-wide analysis by sequencing cDNA derived from RNA. Modern workflows often incorporate pseudoalignment tools (such as…
History & Applications
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rna sequencing expression gene genes used data reads cdna tools include cell methods dna number sequences known different analyses analysis
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| microarrays | instance of | Standard methods | 0.80 | text |
| standard bulk RNA-Seq analysis analyze the expression of RNAs from large populations of cells | instance of | Standard methods | 0.80 | text |
| imprinting or cis-regulatory effects | instance of | This may provide insight into phenomena | 0.80 | text |
| Cufflinks or StringTie to reconstruct contiguous transcript sequences | instance of | tools can be further used by tools | 0.80 | text |
| RNA interference | instance of | but these are often not equivalent due to post transcriptional events | 0.80 | text |
| nonsense-mediated decay.Expression is quantified by counting the number of reads that mapped to each locus in the transcriptome assembly step | instance of | but these are often not equivalent due to post transcriptional events | 0.80 | text |
| RNA-Seq | has application | The | 0.60 | section |
| RNA-Seq | has application | Other | 0.60 | section |
| RNA-Seq | has application | TEs | 0.60 | section |
| RNA-Seq | has application | Neoantigen | 0.60 | section |
| RNA-Seq | related to Alternative splicing | RNA | 0.60 | section |
| RNA-Seq | related to Alternative splicing | There | 0.60 | section |
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