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In genetics, complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA exists in both single-stranded and double-stranded forms and in both natural and engineered forms.
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cdna rna dna mrna reverse used also sequence rna-seq methods expression often transcription protein transcriptase cell complementary via transcribed genome
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| microarrays | instance of | or single nuclei in assays | 0.80 | text |
| qPCR | instance of | or single nuclei in assays | 0.80 | text |
| and RNA-seq.In natural forms | instance of | or single nuclei in assays | 0.80 | text |
| cDNA is produced by retroviruses | instance of | or single nuclei in assays | 0.80 | text |
| phenol-chloroform | instance of | proteins and other cellular components are removed. cDNA is then synthesized through in vitro reverse transcription.RNA purificationRNA is transcribed from genomic DNA in host c… | 0.80 | text |
| silica column | instance of | proteins and other cellular components are removed. cDNA is then synthesized through in vitro reverse transcription.RNA purificationRNA is transcribed from genomic DNA in host c… | 0.80 | text |
| and bead-based RNA extraction methods | instance of | proteins and other cellular components are removed. cDNA is then synthesized through in vitro reverse transcription.RNA purificationRNA is transcribed from genomic DNA in host c… | 0.80 | text |
| DNase | instance of | enzymes | 0.80 | text |
| Proteinase K are used for degradation | instance of | enzymes | 0.80 | text |
| guanidinium isothiocyanate | instance of | RNA integrity is maintained by inactivating RNases with chaotropic agents | 0.80 | text |
| sodium dodecyl sulphate | instance of | RNA integrity is maintained by inactivating RNases with chaotropic agents | 0.80 | text |
| RT-qPCR | instance of | cDNA is commonly generated from mRNA for gene expression analyses | 0.80 | text |
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