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Real-time polymerase chain reaction

A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively…

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Background

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Chemical classification

Fusion temperature analysis

Applications

Bibliography

  • Doi Doi (identifier)
  • PMID PMID (identifier)
  • S2CID S2CID (identifier)
  • Bibcode Bibcode (identifier)
  • JSTOR JSTOR (identifier)
  • PMC PMC (identifier)
  • ISBN ISBN (identifier)

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Real-time polymerase chain reaction

Nodes115
Edges114
Triples82
Avg. degree1.98
Density0.017391
Components1

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Real-time polymerase chain reaction

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related to Bibliography · 75
Real-time polymerase chain reaction → Abramson, Absolute, Acad, Alain, Andrade, Appl, Applications, Applied Microbiology, Archived, Aspects Med, Bengtsson, Bibcode, Bio-Technology, Biology, Biotechnology, Bustin, Caister Academic Press, Current Technology, Detection, DNA

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Important terminology

pcr dna used real-time gene quantification amplification expression fluorescence reaction using polymerase cycle genes mrna fluorescent specific method detection rna

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
SYBR Green will bind to all dsDNA PCR productsinstance ofdsDNA dyes0.80text
including nonspecific PCR productsinstance ofdsDNA dyes0.80text
MAK2 have been shown to have equal or better quantitative performance to standard curve methodsinstance ofMethods0.80text
the visual examination of RNA gelsinstance ofthe most crucial aspect of the process is that the reference gene must be stable.The selection of these reference genes was traditionally carried out in molecular biology using…0.80text
northern blot densitometry or semi-quantitative PCRinstance ofthe most crucial aspect of the process is that the reference gene must be stable.The selection of these reference genes was traditionally carried out in molecular biology using…0.80text
DNA or protein analysis are usually less sensitiveinstance ofAlternatives0.80text
the hepatitis B virusinstance ofof a virus0.80text
Real-time polymerase chain reactionrelated to BibliographyElyse0.60section
Real-time polymerase chain reactionrelated to BibliographyHoude0.60section
Real-time polymerase chain reactionrelated to BibliographyAlain0.60section
Real-time polymerase chain reactionrelated to BibliographyLa PCR0.60section
Real-time polymerase chain reactionrelated to BibliographyPDF0.60section

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