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Single-cell sequencing

Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. For example, in cancer, sequencing the DNA of…

Applications, Genome (DNA) sequencing & Limitations

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Overview

Background

Genome (DNA) sequencing

Methods

Limitations

Applications

DNA methylome sequencing

Transcriptome sequencing (scRNA-seq)

Considerations

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Single-cell sequencing

Nodes102
Edges101
Triples64
Avg. degree1.98
Density0.019608
Components1

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Single-cell sequencing

Top relations

related to Genome (DNA) sequencing · 16
Single-cell sequencing → Advancements, Although SAGs, Cortex, Data, DNA, HyDA, IDBA-UD, Illumina, In, Ion Torrent, SAG, SAGs, Single-cell, Single-cell DNA, Some, SPAdes
related to Limitations · 16
Single-cell sequencing → CNV, CNVs, DNA, Due, FISH, GC, In, MDA, Single-nucleotide, SNPs, The, There, To, Various SNP, WGA-X, With MDA
related to background · 13
Single-cell sequencing → By, DNA, For, In, It, Like, Nature Publishing Group, Recent, RNA, Sanger, Single-cell, The, Usually

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Important terminology

sequencing cell cells single-cell single dna method amplification used genome rna mrna methods also individual mda scrna-seq using types rna-seq

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
receptor tyrosine kinase genesinstance ofCancer scDNAseq is particularly useful for examining the depth of complexity and compound mutations present in amplified therapeutic targets0.80text
circulating tumor cellsinstance ofSingle-cell whole-genome bisulfite sequencing has also been used to study rare but highly active cell types in cancer0.80text
microarraysinstance ofStandard methods0.80text
bulk RNA-seq analyze the RNA expression from large populations of cellsinstance ofStandard methods0.80text
other highly abundant rRNA moleculesinstance ofand size selection to exclude large RNA species0.80text
axonsinstance ofof total RNA is in cellular processes0.80text
dendritesinstance ofof total RNA is in cellular processes0.80text
astrocyte end-feetinstance ofof total RNA is in cellular processes0.80text
and thus not visible to scRNA-seq methods.ApplicationsscRNA-Seq is becoming widely used across biological disciplines including Developmental biologyinstance ofof total RNA is in cellular processes0.80text
Neurologyinstance ofof total RNA is in cellular processes0.80text
Oncologyinstance ofof total RNA is in cellular processes0.80text
Immunologyinstance ofof total RNA is in cellular processes0.80text

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