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Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells, including plant cells. In animal…
Methods, RNA transfection & Overview
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cells cell rna dna used transfected long methods also gene material electroporation phosphate transformation viral eukaryotic genetic molecules may physical
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| Transfection | is a | process of deliberately introducing naked or purified nucleic acids into eukaryotic cells | 0.90 | text |
| Transfection | is a | preferred term | 0.90 | text |
| Transfection | is a | portmanteau of the prefix trans- and the word | 0.90 | text |
| Transfection | is a | process of deliberately introducing RNA molecules longer than about 25nt into living cells | 0.90 | text |
| lipofection | instance of | Chemicals include methods | 0.80 | text |
| which is a lipid-mediated DNA-transfection process utilizing liposome vectors | instance of | Chemicals include methods | 0.80 | text |
| DEAE-dextran or polyethylenimine | instance of | This process has been a preferred method of identifying many oncogenes.Another method is the use of cationic polymers | 0.80 | text |
| N6-methyladenosine | instance of | suggesting that characteristics other than 5'-phosphorylation can influence the immunogenicity of an RNA molecule.Eukaryotic mRNA contains chemically modified nucleotides | 0.80 | text |
| 5-methylcytidine | instance of | suggesting that characteristics other than 5'-phosphorylation can influence the immunogenicity of an RNA molecule.Eukaryotic mRNA contains chemically modified nucleotides | 0.80 | text |
| and 2'-O-methylated nucleotides | instance of | suggesting that characteristics other than 5'-phosphorylation can influence the immunogenicity of an RNA molecule.Eukaryotic mRNA contains chemically modified nucleotides | 0.80 | text |
| Transfection | has method | There | 0.60 | section |
| Transfection | has method | DNA | 0.60 | section |
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