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Protein sequencing

Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide. This may serve to identify the protein or characterize its post-translational modifications. Typically, partial sequencing of a protein provides sufficient information (one or more sequence tags) to identify it with reference to…

Art, Determining amino acid composition & Edman degradation

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Overview

Determining amino acid composition

N-terminal amino acid analysis

C-terminal amino acid analysis

Edman degradation

Identification by mass spectrometry

Predicting from DNA/RNA sequences

Bioinformatics tools

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Protein sequencing

Nodes84
Edges83
Triples21
Avg. degree1.98
Density0.02381
Components1

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Protein sequencing

Top relations

related to Edman degradation · 8
Protein sequencing → Automated Edman, Break, Construct, Determine, Edman, Repeat, Separate, The Edman
has application · 2
Protein sequencing → Such, The
is a · 1
Protein sequencing → practical process of determining the amino acid sequence of all or part of a protein or peptide

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Important terminology

protein amino may sequence acid peptide acids mass sequencing edman degradation used sequences peptides post-translational identification chromatography also modifications spectrometry

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Protein sequencingis apractical process of determining the amino acid sequence of all or part of a protein or peptide0.90text
ortho-phthaldehydeinstance offluorescent derivatives can be formed using reagents0.80text
lysine - for this reason it is necessary to be careful in interpreting chromatograms to ensure that the right spot is choseninstance ofThey all react with amine groups and will therefore also bind to amine groups in the side chains of amino acids0.80text
dansyl chlorideinstance ofand dansyl derivatives0.80text
iodoacetic acid may be necessary to prevent the bonds from re-forming.Separateinstance ofA protecting group0.80text
purify the individual chains of the protein complexinstance ofA protecting group0.80text
if there are more than one.Determine the amino acid composition of each chain.Determine the terminal amino acids of each chain.Break each chain into fragments under 50 amino acids long.Separateinstance ofA protecting group0.80text
purify the fragments.Determine the sequence of each fragment.Repeat with a different pattern of cleavage.Construct the sequence of the overall protein.Digestion into peptide fragmentsPeptides longer than about 50instance ofA protecting group0.80text
cyanogen bromideinstance ofDigestion is done either by endopeptidases such as trypsin or pepsin or by chemical reagents0.80text
collision-induced dissociationinstance offragmentation of peptide ions may occur via a variety of mechanisms0.80text
UniProtinstance ofand have led to the generation of large databases of protein sequences0.80text
Protein sequencinghas applicationThe0.60section

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