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Column chromatography

Column chromatography is a chromatography method used in analytical chemistry to separate the individual components (analytes) of a mixture. The mixture is carried by a solvent (the eluent) to form the mobile phase. The mobile phase moves through a column packed with solid particles (the stationary phase). The components adsorb to the stationary phase at…

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Overview

Column preparation

Stationary phase

Mobile phase (eluent)

Low pressure liquid chromatography

Column chromatogram resolution calculation

Column adsorption equilibrium

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Column chromatography

Nodes53
Edges52
Triples41
Avg. degree1.96
Density0.037736
Components1

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Column chromatography

Top relations

related to Low pressure liquid chromatography · 12
Column chromatography → Biotage, Buchi, Column, HPLC, Interchim, LPLC, Many, Such, Teledyne Isco, They, Typically, UV
related to Stationary phase · 7
Column chromatography → Cellulose, EBA, In, It, RP, The, This
related to Column chromatogram resolution calculation · 4
Column chromatography → For, Prior, The, Typically
see also · 3
Column chromatography → Fast, FPLC, HPLC
related to External links · 2
Column chromatography → Flash Column Chromatography Guide, Radial Flow Chromatography
is a · 1
Column chromatography → chromatography method used in analytical chemistry to separate the individual components

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Important terminology

column chromatography phase stationary eluent different sample used mixture mobile time resolution components concentration solvent liquid binding separate compounds chromatogram

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Column chromatographyis achromatography method used in analytical chemistry to separate the individual components0.90text
a gradient pumpinstance ofsystems0.80text
sample injection portsinstance ofsystems0.80text
a UV detectorinstance ofsystems0.80text
a fraction collector to collect the eluentinstance ofsystems0.80text
but operating at a lower pressureinstance ofsystems0.80text
a spectrophotometer or mass spectrometer so that the concentration of the separated samples in the sample solution mixture can be determined.For exampleinstance ofthe samples that are eluted from the column pass through a detector0.80text
if you were to separate two different proteins with different binding capacities to the column from a solution sampleinstance ofthe samples that are eluted from the column pass through a detector0.80text
a good type of detector would be a spectrophotometer using a wavelength of 280 nminstance ofthe samples that are eluted from the column pass through a detector0.80text
proteinsinstance ofEven smaller particles0.80text
carbohydratesinstance ofEven smaller particles0.80text
metal ionsinstance ofEven smaller particles0.80text

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    Min side: 3
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