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A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of the transcriptome of the organism and are stored as a "library." cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism.…
Applications, CDNA Library Construction & Cloning of cDNA
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cdna mrna dna libraries library produced genomic introns eukaryotic used cloning primer therefore information cells found expressed reverse tail site
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| CDNA library | is a | combination of cloned cDNA | 0.90 | text |
| trizol extraction | instance of | Several methods exist for purifying RNA | 0.80 | text |
| column purification | instance of | Several methods exist for purifying RNA | 0.80 | text |
| having a poly-A tail can be exploited where only mRNA sequences containing said feature will bind | instance of | and features of mRNA | 0.80 | text |
| CDNA library | related to cDNA construction | Once | 0.60 | section |
| CDNA library | related to cDNA construction | RNA | 0.60 | section |
| CDNA library | related to cDNA construction | The | 0.60 | section |
| CDNA library | related to cDNA construction | DNA | 0.60 | section |
| CDNA library | related to cDNA construction | This | 0.60 | section |
| CDNA library | related to cDNA construction | RNA-DNA | 0.60 | section |
| CDNA library | related to cDNA construction | To | 0.60 | section |
| CDNA library | related to cDNA construction | RNAse | 0.60 | section |
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