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Cloning vector

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium. The vector contains features that allow for the convenient insertion of…

Features of a cloning vector, Types of cloning vectors & Overview

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Features of a cloning vector

Molecular biologyE. coliOrigin of replicationColE1Shuttle vector
49 related topics

Types of cloning vectors

PBR322PUC19M13 bacteriophagePhagemidsPBluescript
19 related topics

Overview

DNACloningVirusCellPlasmids
18 related topics

Topics to explore

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Overview

Features of a cloning vector

Types of cloning vectors

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Map overview Semantic statistics

Cloning vector

Nodes90
Edges89
Triples65
Avg. degree1.98
Density0.022222
Components1

How this topic connects Entity context

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Cloning vector

Top relations

related to Cloning site · 14
Cloning vector → All, Another, DNA, Gateway, Gibson, In, MCS, Other, PCR, PCR-amplified, The, This, TOPO, TOPO-activated
related to Plasmid · 11
Cloning vector → DNA, However, M13, Many, Most, One, Other, Plasmids, Some, These, They
related to Elements for expression · 8
Cloning vector → Cloning, DNA, Promoter, RBS, Some, T7, The, Where
related to Bacteriophage · 7
Cloning vector → DNA, In, Insertion, M13, The, There, This
related to Features of a cloning vector · 6
Cloning vector → All, For, Others, Some, The ColE1, Thus
related to Reporter gene · 5
Cloning vector → Examples, GFP, MCS, Reporter, Such
is a · 1
Cloning vector → small piece of DNA that can be stably maintained in an organism
related to Types of cloning vectors · 1
Cloning vector → Large

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Important terminology

dna cloning vectors vector may used gene example plasmids coli cells cloned fragment plasmid also genes features artificial expression yeast

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Cloning vectoris asmall piece of DNA that can be stably maintained in an organism0.90text
yeast may be usedinstance ofand other organisms0.80text
resistance to zeocininstance ofalthough some selectable markers0.80text
hygromycin B are effective in different cell typesinstance ofalthough some selectable markers0.80text
blue-white selection are used.Cloning vectors without promoterinstance ofThe presence of a promoter is necessary when screening techniques0.80text
RBS for the cloned DNA sequence are sometimes usedinstance ofThe presence of a promoter is necessary when screening techniques0.80text
for example when cloning genes whose products are toxic to E. coli cellsinstance ofThe presence of a promoter is necessary when screening techniques0.80text
BCCM/GeneCorner.BacteriophageThe bacteriophages used for cloning are the λ phageinstance ofsuch repositories include Addgene and public collections0.80text
M13 phageinstance ofsuch repositories include Addgene and public collections0.80text
in the Human Genome Projectinstance ofThey are useful in cloning larger DNA fragments as required in mapping genomes0.80text
BCCM/GeneCornerinstance ofsuch repositories include Addgene and public collections0.80text
pUC19 usually include a system for detecting the presence of a cloned DNA fragmentinstance ofexample of the blue/white screenMany general purpose vectors0.80text

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