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DNA extraction

The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA…

Standards, Basic procedure & Detection of DNA

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Overview

Basic procedure

Method selection

Special types

Detection of DNA

High-molecular-weight DNA extraction method

Quality control

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Map overview Semantic statistics

DNA extraction

Nodes79
Edges78
Triples56
Avg. degree1.97
Density0.025316
Components1

How this topic connects Entity context

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DNA extraction

Top relations

related to Common chemicals · 13
DNA extraction → Detergents, DNA, EDTA, Ethanol, NaCl, Phenol, Protease, Proteinase, Salt, SDS, The, Tris-HCL, Tween-20
related to Method selection · 9
DNA extraction → Chelex, DNA, It, Organic, Several, Some, These, Though, When
related to What does it deliver? · 6
DNA extraction → DNA, FISH, Fluorescence In Situ Hybridization, It, The, These
related to DNA storage · 5
DNA extraction → DNA, One, TE, The DNA, Tris-EDTA
related to External links · 3
DNA extraction → DNA, Extraction Virtual Lab, How
is a · 2
DNA extraction → isolation of all extrachromosomal DNA in a mammalian cell, process of isolating DNA from the cells of an organism isolated from a sample

Important terminology Word statistics

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Important terminology

dna used extraction method proteins sample precipitation centrifugation ethanol concentration solution cells methods pcr buffer adding purification specific lysis storage

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
DNA extractionis aprocess of isolating DNA from the cells of an organism isolated from a sample0.90text
DNA extractionis aisolation of all extrachromosomal DNA in a mammalian cell0.90text
bloodinstance oftypically a biological sample0.80text
salivainstance oftypically a biological sample0.80text
or tissueinstance oftypically a biological sample0.80text
PCRinstance ofThe purified DNA can then be used for downstream applications0.80text
sequencinginstance ofThe purified DNA can then be used for downstream applications0.80text
or cloninginstance ofThe purified DNA can then be used for downstream applications0.80text
broken proteinsinstance ofto make debris0.80text
lipidsinstance ofto make debris0.80text
and RNA clump together.Centrifugation of the solutioninstance ofto make debris0.80text
which separates the clumped cellular debris from the DNA.DNA purification from detergentsinstance ofto make debris0.80text

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