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An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in…
Applications, Elements & Expression/Production systems
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expression used protein may vectors vector gene proteins also production coli promoter cell use expressed host plant systems produced mammalian
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| Expression vector | is a | efficient production of protein | 0.90 | text |
| Expression vector | is a | production of insulin | 0.90 | text |
| a ribosomal binding site | instance of | the correct translation initiation sequence | 0.80 | text |
| start codon | instance of | the correct translation initiation sequence | 0.80 | text |
| a termination codon | instance of | the correct translation initiation sequence | 0.80 | text |
| and a transcription termination sequence | instance of | the correct translation initiation sequence | 0.80 | text |
| IPTG | instance of | meaning that protein synthesis is only initiated when required by the introduction of an inducer | 0.80 | text |
| glutathione S-transferase or maltose-binding protein | instance of | or a fusion partners | 0.80 | text |
| green fluorescent protein may act as a reporter gene for the identification of successful cloned genes | instance of | Other fusion proteins | 0.80 | text |
| or they may be used to study protein expression in cellular imaging.Other ElementsThe expression vector is transformed or transfected into the host cell for protein synthesis | instance of | Other fusion proteins | 0.80 | text |
| the periplasmic space of bacteria | instance of | and integrase sites for chromosomal integration .Some vectors may include targeting sequence that may target the expressed protein to a specific location | 0.80 | text |
| or they may be used to study protein expression in cellular imaging | instance of | Other fusion proteins | 0.80 | text |
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