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Bisulfite sequencing

Bisulfite sequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied. In animals it predominantly involves the addition of a methyl group to the carbon-5 position of…

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Limitations

Oxidative bisulfite sequencing

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Bisulfite sequencing

Nodes80
Edges79
Triples73
Avg. degree1.98
Density0.025
Components1

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Bisulfite sequencing

Top relations

has method · 17
Bisulfite sequencing → All, Alternative, Bisulfite, C-to-U-converted, Combined Bisulphite Restriction Analysis, CpG, DNA, Ideally, MeDIP, Methodologies, Microarray-based, Oligonucleotide, One, Other, The, The Illumina Methylation Assay, To
related to Oxidative bisulfite sequencing · 16
Bisulfite sequencing → Another, Bisulfite, Chicago, Chuan He, DNA, In, Levels, Methylcytosine, PCR, TAB-Seq, Tet, Tet-assisted, The, The Tet, To, University
has treatment · 14
Bisulfite sequencing → APOBEC, Distinction, DNA, Given, In, Instead, NEBNext Enzymatic Methyl-seq, New England Biolabs, PCR, Techniques, The, Therefore, This, Thus
has application · 9
Bisulfite sequencing → DNA, Epigenomic, Human Genome Project, One's, Restriction, Since, Such, The, This
related to Incomplete conversion · 7
Bisulfite sequencing → Bisulfite, DNA, Embedding, If, Incomplete, It, Only
related to 5-Hydroxymethylcytosine · 5
Bisulfite sequencing → Bisulfite, DNA, Hydroxymethylcytosine, Therefore, This
is a · 1
Bisulfite sequencing → degradation of DNA that takes place concurrently with the conversion
see also · 1
Bisulfite sequencing → Reduced

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Important terminology

dna bisulfite methylation sequencing pcr analysis primer used also cpg method conversion treatment using sites sequence based resulting cytosines methods

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Bisulfite sequencingis adegradation of DNA that takes place concurrently with the conversion0.90text
temperatureinstance ofIt is important to ensure that reaction parameters0.80text
salt concentration are suitable to maintain the DNA in a single-stranded conformationinstance ofIt is important to ensure that reaction parameters0.80text
allow for complete conversioninstance ofIt is important to ensure that reaction parameters0.80text
Bisulfite sequencinghas applicationThe0.60section
Bisulfite sequencinghas applicationDNA0.60section
Bisulfite sequencinghas applicationRestriction0.60section
Bisulfite sequencinghas applicationHuman Genome Project0.60section
Bisulfite sequencinghas applicationThis0.60section
Bisulfite sequencinghas applicationSince0.60section
Bisulfite sequencinghas applicationEpigenomic0.60section
Bisulfite sequencinghas applicationOne's0.60section

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