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Matrix-assisted laser desorption/ionization

In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy-absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and carbohydrates) and various organic molecules…

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Matrix

Instrumentation

Ionization mechanism

Applications

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Matrix-assisted laser desorption/ionization

Nodes125
Edges124
Triples40
Avg. degree1.98
Density0.016
Components1

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Matrix-assisted laser desorption/ionization

Top relations

related to Atmospheric pressure · 16
Matrix-assisted laser desorption/ionization → AP, AP-MALDI, Atmospheric, Besides, DNA, ESI, In, Ionization, MALDI, MS, Multiple, PNA, Popular, RNA, The, The AP-MALDI

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Important terminology

maldi matrix laser ionization used mass molecules ion also ions analyte spectrometry protein acid tof sample pressure desorption source maldi-tof

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
DNAinstance ofbiopolymers0.80text
proteinsinstance ofbiopolymers0.80text
peptidesinstance ofbiopolymers0.80text
carbohydratesinstance ofbiopolymers0.80text
acetonitrileinstance ofoften in a mixture of highly purified water and an organic solvent0.80text
trifluoroacetic acidinstance ofA counter ion source0.80text
chlorophyllinstance ofThe most widely used application for these matrices is studying porphyrin-like compounds0.80text
the FT-ICR instruments as well as more high-throughput instrumentsinstance ofThe mass spectrometry field has expanded into requiring ultrahigh resolution mass spectrometry0.80text
nitrogen lasersinstance ofSoft laser desorption or Ion source.LaserMALDI techniques typically employ the use of UV lasers0.80text
quadrupolesinstance ofwhich provide only smaller m/z detection ranges0.80text
nitrogen lasersinstance ofLaserMALDI techniques typically employ the use of UV lasers0.80text
bacteria or fungiinstance ofotherwise the sample will precipitate out of solution.MicrobiologyMALDI-TOF spectra are often used for the identification of microorganisms0.80text

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