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Western blot normalization

Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages. The overall goal of normalization is to minimize effects arising from variations in experimental errors, such…

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Housekeeping protein controls

Housekeeping genesΒ-ActinGAPDHHPRT1RPLP1
8 related topics

Total protein normalization

Ponceau SCoomassie brilliant blueSignal-to-noise ratioCharge-coupled deviceCamera
7 related topics

Procedure

Loading internal control
1 related topics

Overview

Western blotProteinGel
3 related topics

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Overview

Procedure

Housekeeping protein controls

Total protein normalization

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Map overview Semantic statistics

Western blot normalization

Nodes24
Edges23
Triples12
Avg. degree1.92
Density0.083333
Components1

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Important terminology

protein normalization blot proteins western gel stain-free fluorescent stains control loading data total intensity range across controls antibodies housekeeping target

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Ponceau Sinstance ofNon-uniformity in the image can result in inaccurate normalization.Pre-antibody stainsAnionic dyes0.80text
Coomassie brilliant blueinstance ofNon-uniformity in the image can result in inaccurate normalization.Pre-antibody stainsAnionic dyes0.80text
and fluorescent dyes like Sypro Rubyinstance ofNon-uniformity in the image can result in inaccurate normalization.Pre-antibody stainsAnionic dyes0.80text
Deep Purpleinstance ofNon-uniformity in the image can result in inaccurate normalization.Pre-antibody stainsAnionic dyes0.80text
are used before antibodies are added because they do not affect downstream immunodetection.Ponceau S is a negatively charged reversible dye that stains proteins a reddish pink colorinstance ofNon-uniformity in the image can result in inaccurate normalization.Pre-antibody stainsAnionic dyes0.80text
is removed easily by washing in waterinstance ofNon-uniformity in the image can result in inaccurate normalization.Pre-antibody stainsAnionic dyes0.80text
Ponceau Sinstance ofPre-antibody stainsAnionic dyes0.80text
Coomassie brilliant blueinstance ofPre-antibody stainsAnionic dyes0.80text
and fluorescent dyes like Sypro Rubyinstance ofPre-antibody stainsAnionic dyes0.80text
Deep Purpleinstance ofPre-antibody stainsAnionic dyes0.80text
are used before antibodies are added because they do not affect downstream immunodetection.Ponceau S is a negatively charged reversible dye that stains proteins a reddish pink colorinstance ofPre-antibody stainsAnionic dyes0.80text
is removed easily by washing in waterinstance ofPre-antibody stainsAnionic dyes0.80text

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