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Polyacrylamide gel electrophoresis

Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Electrophoretic mobility is a function of the length, conformation, and charge of the…

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Chemical ingredients and their roles

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Polyacrylamide gel electrophoresis

Nodes106
Edges105
Triples11
Avg. degree1.98
Density0.018868
Components1

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Polyacrylamide gel electrophoresis

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Polyacrylamide gel electrophoresis → powerful tool used to analyze RNA samples

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gel proteins used acrylamide electrophoresis protein may polyacrylamide sds gels mobility molecules size also molecular page native ratio pore weight

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SubjectPredicateObjectConfidenceSrc
Polyacrylamide gel electrophoresisis apowerful tool used to analyze RNA samples0.90text
oligonucleotides may also be used as analytes.The sample to analyze is optionally mixed with a chemical denaturant if so desiredinstance ofSynthetic biomolecules0.80text
usually SDS for proteins or urea for nucleic acidsinstance ofSynthetic biomolecules0.80text
DISC SDS-PAGE the pH values within the gel may vary to change the average charge of the counterions during the run to improve resolutioninstance ofIn applications0.80text
hydrogen bondsinstance ofis a chaotropic agent that increases the entropy of the system by interfering with intramolecular interactions mediated by non-covalent forces0.80text
van der Waals forcesinstance ofis a chaotropic agent that increases the entropy of the system by interfering with intramolecular interactions mediated by non-covalent forces0.80text
SYBR Greeninstance ofmany researchers opt to use stains0.80text
SYBR Safe which are safer alternatives to EtBrinstance ofmany researchers opt to use stains0.80text
a nitrocelluloseinstance ofmanipulable membrane0.80text
nyloninstance ofmanipulable membrane0.80text
or polyvinylidene difluorideinstance ofmanipulable membrane0.80text

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