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Immunoprecipitation

Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled…

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Immunoprecipitation

Nodes62
Edges61
Triples106
Avg. degree1.97
Density0.032258
Components1

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Immunoprecipitation

Top relations

related to Chromatin immunoprecipitation (ChIP) · 18
Immunoprecipitation → Alternatively, At, By, ChIP, ChIP-chip, ChIP-on-chip, ChIP-sequencing, Chromatin, DNA, DNA-binding, DTBP, Following, HCl, PCR, Previously, Sometimes, The, This
related to Steps · 18
Immunoprecipitation → After, Analyze, Antibody, Continue, Elute, Incubate, IP, Lyse, MALDI, Pre-clear, Precipitate, SDS, SDS-PAGE, Spin, This, Transfer, Wash, Western
related to RNP immunoprecipitation (RIP and CLIP) · 11
Immunoprecipitation → CLIP, In CLIP, In RIP, RIP, RNA, RNA-binding, RNAs, RNPs, RT-PCR, SDS-PAGE, UV
related to External links · 10
Immunoprecipitation → Analysis, Chromatin, Co-IP, Immunoprecipitation Methodology Co-Immunoprecipitation, Introduction, Medicine Medical Subject Headings, MeSH, National Library, Proteins Using Immunoprecipitation, Technical
related to Superparamagnetic beads · 10
Immunoprecipitation → Although, Commercially, Invitrogen, IP, Millipore, Monodisperse, Polydisperse, Thermo Scientific, Unlike, While
related to Tagged proteins · 10
Immunoprecipitation → Examples, FLAG-tag, GFP, GST, IP, One, S-transferase, The, To, While
related to background · 8
Immunoprecipitation → After, At, IP, Once, Separation, The, This, When
related to Agarose · 7
Immunoprecipitation → Because, Historically, In, IP, It, The, While
related to Agarose vs. magnetic beads · 5
Immunoprecipitation → Additional, Claims, IP, Proponents, The
related to Protein complex immunoprecipitation (Co-IP) · 3
Immunoprecipitation → By, Co-IP, This

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Important terminology

protein beads agarose antibody binding proteins magnetic ip using used may capacity complex antibodies use sample dna method particular complexes

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Immunoprecipitationis agreat difficulty in generating an antibody that specifically targets a single known protein0.90text
Immunoprecipitationis anewer approach that is gaining in popularity as an alternative to agarose beads for IP applications0.90text
dimethyl 3instance ofalthough it is sometimes advantageous to use a more defined and consistent crosslinker0.80text
3instance ofalthough it is sometimes advantageous to use a more defined and consistent crosslinker0.80text
superparamagnetic microbeads or on microscopic agaroseinstance ofare immobilized on a solid-phase substrate0.80text
Immunoprecipitationhas methodThe0.60section
Immunoprecipitationrelated to AgaroseHistorically0.60section
Immunoprecipitationrelated to AgaroseThe0.60section
Immunoprecipitationrelated to AgaroseIP0.60section
Immunoprecipitationrelated to AgaroseBecause0.60section
Immunoprecipitationrelated to AgaroseIn0.60section
Immunoprecipitationrelated to AgaroseIt0.60section

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