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BLESS

BLESS, also known as breaks labeling, enrichment on streptavidin and next-generation sequencing, is a method used to detect genome-wide double-strand DNA damage. In contrast to chromatin immunoprecipitation (ChIP)-based methods of identifying DNA double-strand breaks (DSBs) by labeling DNA repair proteins, BLESS utilizes biotinylated DNA linkers to…

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Overview

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Computational analysis

Advantages

Limitations

Alternative methods

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Map overview Semantic statistics

BLESS

Nodes53
Edges52
Triples42
Avg. degree1.96
Density0.037736
Components1

How this topic connects Entity context

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BLESS

Top relations

has application · 9
BLESS → CRISPR-Cas9, DNA, DSB-mapping, DSBs, For, Since BLESS, TALEN, These, While
related to Advantages · 9
BLESS → Because, DNA, DSB-proxy, DSBs, For, H2A, Labeling, This, Use
related to Limitations · 8
BLESS → Because, BLISS, Compared, DNA, Due, However, PCR, To
related to PCR amplification and sequencing · 7
BLESS → Although, DNA, I-SceI, PCR, Sanger, The, XhoI
related to Computational analysis · 6
BLESS → DNA, DSBs, Instant Sequencing, The, The BLESS, To
related to External links · 1
BLESS → BLESS Supporting Website

Important terminology Word statistics

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Important terminology

dna dsbs breaks biotinylated linker labeling streptavidin genome enrichment sequencing methods using also beads distal analysis biotin specific sample double-stranded

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
yeast cellsinstance ofA limitation of the original BLESS method is that it is problematic in application to smaller cells0.80text
CRISPR-Cas9 or TALENinstance ofspecific DSB-mapping methods like BLESS are useful for breakome surveys.DSBs can be artificially induced using genome editing technologies0.80text
BLESShas applicationWhile0.60section
BLESShas applicationDNA0.60section
BLESShas applicationFor0.60section
BLESShas applicationDSB-mapping0.60section
BLESShas applicationDSBs0.60section
BLESShas applicationCRISPR-Cas90.60section
BLESShas applicationTALEN0.60section
BLESShas applicationThese0.60section
BLESShas applicationSince BLESS0.60section
BLESSrelated to AdvantagesUse0.60section

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