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Strep-tag

The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin, and can be N- or C-…

Applications, Strep-tag applications & The Strep-tag principle

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Strep-tag applications

Horseradish peroxidaseAlkaline phosphataseGreen fluorescent proteinColony blotDot blot
13 related topics

The Strep-tag principle

His-tagFLAG-tagRecombinantCDNAGene
14 related topics

Development and biochemistry of the Strep-tag

Streptomyces avidiniiBiotinMolecular biologyBiotechnologyProteome
5 related topics

Overview

ProteinsAffinity chromatographyPeptideAmino acidsTrp
14 related topics

Topics to explore

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Overview

Development and biochemistry of the Strep-tag

The Strep-tag principle

Strep-tag applications

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Map overview Semantic statistics

Strep-tag

Nodes51
Edges50
Triples36
Avg. degree1.96
Density0.039216
Components1

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Strep-tag

Top relations

related to The Strep-tag principle · 16
Strep-tag → After, Finally, FLAG-tag, For, HABA, His-tag, In, Just, PBS, Strep-Tactin, Strep-Tactin's, The, Then, Therefore, This, To
has application · 11
Strep-tag → Also, AP, Depending, Following, GFP, HRP, If, Strep-Tactin, The, The Strep-tag, This
related to Development and biochemistry of the Strep-tag · 9
Strep-tag → Because, Further, Over, Strep-Tactin, Streptavidin, Streptavidin's, Streptomyces, The, The Strep-tag

Important terminology Word statistics

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Important terminology

proteins protein strep-tactin streptavidin affinity system step also purification specifically high physiological peptide detection interaction conditions functional desthiobiotin recombinant used

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Strep-taghas applicationThe Strep-tag0.60section
Strep-taghas applicationThe0.60section
Strep-taghas applicationAlso0.60section
Strep-taghas applicationDepending0.60section
Strep-taghas applicationStrep-Tactin0.60section
Strep-taghas applicationHRP0.60section
Strep-taghas applicationAP0.60section
Strep-taghas applicationGFP0.60section
Strep-taghas applicationIf0.60section
Strep-taghas applicationThis0.60section
Strep-taghas applicationFollowing0.60section
Strep-tagrelated to Development and biochemistry of the Strep-tagStreptavidin0.60section

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