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Affinity chromatography

Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. The specific type of binding interaction depends on the biomolecule of interest; antigen and antibody, enzyme and substrate, receptor and ligand, or protein and…

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Overview

Principle

Batch and column setups

Specific uses

Weak affinity chromatography

History

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Map overview Semantic statistics

Affinity chromatography

Nodes64
Edges63
Triples76
Avg. degree1.97
Density0.03125
Components1

How this topic connects Entity context

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Affinity chromatography

Top relations

related to Recombinant proteins · 15
Affinity chromatography → CL7, Colicin E7, During, For, GST, His, Histidine, Im7, Immunity Protein, MBP, Possibly, Protein, Proteins, S-transferase, The
related to Lectins · 11
Affinity chromatography → Although, Another, Con A-Sepharose, D-glucose, D-mannose, D-N-acetyl-glucosamine, Lectin, Lectins, Some, The, WGA-agarose
related to Immunoaffinity · 9
Affinity chromatography → Another, Elution, For, GST, GST-fusion, If, Immunoaffinity Chromatography, The, This
related to Immobilized metal ion affinity chromatography · 6
Affinity chromatography → DNA, IMAC, Immobilized, Many, Methods, This
related to Principle · 6
Affinity chromatography → Affinity, In, Molecules, Target, The, Types
related to Weak affinity chromatography · 6
Affinity chromatography → Affinity, PPI, The, The WAC, WAC, Weak
related to Specialty · 5
Affinity chromatography → Another, D-galactopyranosyl, For, Galactosidase, This
related to External links · 4
Affinity chromatography → Affinity Chromatography Principle, Note, Procedure And Advance Detailed, What
is a · 3
Affinity chromatography → form of affinity chromatography where lectins are used to separate components within the sample, method of separating a biomolecule from a mixture, purification of specific proteins using a gel matrix that is unique to a specific protein
related to history · 3
Affinity chromatography → Affinity, Meir Wilchek, Pedro Cuatrecasas

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Important terminology

affinity chromatography protein used proteins purification specific needed column immobilized serum ligand citation antibodies binding phase matrix using agarose elution

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Affinity chromatographyis amethod of separating a biomolecule from a mixture0.90text
Affinity chromatographyis aform of affinity chromatography where lectins are used to separate components within the sample0.90text
Affinity chromatographyis apurification of specific proteins using a gel matrix that is unique to a specific protein0.90text
Affinity chromatographypart ofa larger suite of techniques used in chemoproteomics based drug target identification0.85text
agarose or polyacrylamideinstance ofusually a polymer0.80text
agaroseinstance ofThe protein can then be covalently coupled to a solid support0.80text
used as an affinity ligand in purifications of antibody from immune seruminstance ofThe protein can then be covalently coupled to a solid support0.80text
glycine pH 2.8instance ofElution of the antibodies of interest is most often achieved using a low pH buffer0.80text
Affinity chromatographyrelated to External linksAffinity Chromatography Principle0.60section
Affinity chromatographyrelated to External linksProcedure And Advance Detailed0.60section
Affinity chromatographyrelated to External linksNote0.60section
Affinity chromatographyrelated to External linksWhat0.60section

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