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ELISA

The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzə/, /ˌiːˈlaɪzə/) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the…

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History & Applications

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MeSH
D004797

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Overview

Principle

History

Types

Commonly used enzymatic markers

Applications

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ELISA

Nodes78
Edges77
Triples88
Avg. degree1.97
Density0.025641
Components1

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ELISA

Top relations

related to history · 16
ELISA → ABTS, As, Before, However, In, Jerker Porath, Pierce, Radioimmunoassay, Rosalyn Sussman Yalow, Since, Solomon Berson, Stratis Avrameas, This, TMB, When, Wide
related to External links · 11
ELISA → An, Arizona, Assay Genie, ELISA Activity, ELISA Assay Principle, HIV, Introduction, Medicine Medical Subject Headings, MeSH, National Library, University
has application · 9
ELISA → Because, HIV, If, In, It, The, The ELISA, This, West Nile
related to Commonly used enzymatic markers · 9
ELISA → ABTS, Azinobis, Disodium Salt, HRP, Nitrophenyl Phosphate, OPD, PNPP, The, TMB
related to Indirect · 8
ELISA → Antibodies, Scavenger, Scavenger Antigens, The, The Scavenger, These, This, Unlabeled
related to Principle · 6
ELISA → As, Even, In ELISA, Since, The, This
related to Sandwich · 5
ELISA → Any, Enzyme-linked, Fc, The, This
related to Technology · 4
ELISA → Building, ELISA's, However, This
used for · 2
ELISA → detecting HIV, research often needs validation
related to Competitive · 2
ELISA → The, Unlabeled

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Important terminology

antibody antigen antibodies sample enzyme used plate signal detection well test added specific color use surface binding assay reaction analyte

Entity relationships Subject–Predicate–Object triples

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SubjectPredicateObjectConfidenceSrc
ELISAMeSHD0047971.00infobox
reflectometryinstance ofanalysis involves reading of a dried strip by methods0.80text
does not need a reaction containment chamber to prevent spillover or mixing between samples.As a heterogenous assayinstance ofanalysis involves reading of a dried strip by methods0.80text
ELISA separates some components of the analytical reaction mixture by adsorbing certain components onto a solid phase which is physically immobilizedinstance ofanalysis involves reading of a dried strip by methods0.80text
protein kinasesinstance ofThis method allows for the precise quantification of low-abundance proteins and the activity of critical enzymes0.80text
telomerasesinstance ofThis method allows for the precise quantification of low-abundance proteins and the activity of critical enzymes0.80text
which are often below the detection threshold of conventional ELISA.ApplicationsThe enhanced sensitivity of eSimoa is crucial for earlyinstance ofThis method allows for the precise quantification of low-abundance proteins and the activity of critical enzymes0.80text
accurate biomarker detection in clinical diagnosticsinstance ofThis method allows for the precise quantification of low-abundance proteins and the activity of critical enzymes0.80text
facilitating better disease monitoringinstance ofThis method allows for the precise quantification of low-abundance proteins and the activity of critical enzymes0.80text
managementinstance ofThis method allows for the precise quantification of low-abundance proteins and the activity of critical enzymes0.80text
which are often below the detection threshold of conventional ELISAinstance ofThis method allows for the precise quantification of low-abundance proteins and the activity of critical enzymes0.80text
ELISAused forresearch often needs validation0.80text

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