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MALBAC

Multiple Annealing and Looping Based Amplification Cycles (MALBAC) is a quasilinear whole genome amplification method. Unlike conventional DNA amplification methods that are non-linear or exponential (in each cycle, DNA copied can serve as template for subsequent cycles), MALBAC utilizes special primers that allow amplicons to have complementary ends and…

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Technological platform

Applications

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Limitations

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Map overview Semantic statistics

MALBAC

Nodes31
Edges30
Triples77
Avg. degree1.94
Density0.064516
Components1

How this topic connects Entity context

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MALBAC

Top relations

related to Experimental workflow · 18
MALBAC → After, At, Bst DNA Polymerase, By, DNA, Extension, For, Large Fragment, Looping, Melting, PCR, Quenching, Regular PCR, Repeat, Single-cell, The, The MALBAC, This
has application · 13
MALBAC → At, By, CNVs, December, DNA, In, It, Moreover, Science, SNPs, SW4802, The, This
related to Low incidence of false positive and false negative mutations · 10
MALBAC → Allelic, DNA, False, In, MDA, Single, SNP, SNPs, The, This
related to MALBAC primers · 8
MALBAC → After, DNA, GAG, GTG AGT GAT GGT, TGA GGT AGT GTG, The, These, This
related to External links · 6
MALBAC → Genomics, Looping-based Amplification Cycles, Multiple Annealing, NCBI, WGA, Whole Genome Amplification
related to Limitations · 6
MALBAC → Although MALBAC, DNA, Due, Genome, In, SNPs
related to Reduces amplification bias and increases genome coverage · 6
MALBAC → Accordingly, DNA, MDA, PCR, The, These
related to Strand displacement · 4
MALBAC → Bacillus, Bst, DNA, This
related to Advantages · 2
MALBAC → DNA, Some
related to Requires very little template DNA · 2
MALBAC → DNA, Only

Important terminology Word statistics

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Important terminology

dna amplification single sequencing primers template cell genome used amplicons cycles rate false bias polymerase cells coverage sequence amplified genomic

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
one derived from Bacillus stearothermophilusinstance ofA DNA polymerase0.80text
MALBAChas applicationDNA0.60section
MALBAChas applicationThis0.60section
MALBAChas applicationAt0.60section
MALBAChas applicationMoreover0.60section
MALBAChas applicationCNVs0.60section
MALBAChas applicationSNPs0.60section
MALBAChas applicationIn0.60section
MALBAChas applicationIt0.60section
MALBAChas applicationDecember0.60section
MALBAChas applicationScience0.60section
MALBAChas applicationSW48020.60section

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