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Phage display

Phage display is a laboratory technique for the study of protein interactions that uses bacteriophages (viruses that infect bacteria) to produce and "display" the proteins on their surfaces. Since the proteins remain attached to the surface of the phage, it is possible to isolate the phages displaying desirable proteins from among very large collections…

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Overview

History

Principle

Applications

General protocol

Bioinformatics resources and tools

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Map overview Semantic statistics

Phage display

Nodes72
Edges71
Triples85
Avg. degree1.97
Density0.027778
Components1

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Phage display

Top relations

related to Antibody maturation in vitro · 23
Phage display → Antibody, Barbas, Breitling, Carlos, Fab, Frank Breitling, German Cancer Research Centre, Greg Winter, HIV-1, In, Initial, John McCafferty, Molecular Biology, MRC Laboratory, Patent CA, Phage, Richard Lerner, Scripps Research Institute, Stefan Dübel, The
has application · 17
Phage display → Applications, As, Competing, Dinutuximab, DNA, FDA-approved, GD2, GD2-positive, In, It, M13, Phage, Recently, T-Cells, The, These, Upon
related to General protocol · 10
Phage display → Attached, Below, DNA, Following, Many, Phage-displaying, Steps, Target, The, This
related to T7 phages · 9
Phage display → C-terminus, Ff, However, In, In T7, M13, Some, T7, The
related to history · 8
Phage display → George, George Smith, In, Jamie Scott, Phage, Smith, Stephen Parmley, This
related to Principle · 7
Phage display → DNA, In, M13, Phage, Specifically, The, Those
is a · 3
Phage display → attractive alternative to the yeast-2-hybrid method for the discovery of interacting proteins and peptides due to its high throughput capability. pVI has been used preferentiall…, laboratory technique for the study of protein interactions that uses bacteriophages, useful tool in drug discovery
related to Bioinformatics resources and tools · 3
Phage display → Databases, Some, Users

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Important terminology

phage protein display proteins piii used phages coat dna using antibody libraries selection antibodies pviii also surface interest bacteria sequence

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Phage displayis alaboratory technique for the study of protein interactions that uses bacteriophages0.90text
Phage displayis auseful tool in drug discovery0.90text
Phage displayis aattractive alternative to the yeast-2-hybrid method for the discovery of interacting proteins and peptides due to its high throughput capability. pVI has been used preferentiall…0.90text
antibodies for therapeutic protein engineering.Smithinstance ofThe Scripps Research Institute with Richard Lerner and Carlos Barbas and the German Cancer Research Center with Frank Breitling and Stefan Dübel for display of proteins0.80text
Winter were awarded a half share of the 2018 Nobel Prize in chemistry for their contribution to developing phage displayinstance ofThe Scripps Research Institute with Richard Lerner and Carlos Barbas and the German Cancer Research Center with Frank Breitling and Stefan Dübel for display of proteins0.80text
the adoptive cell transfer approachinstance ofphage display has also been used in the context of cancer treatments -0.80text
selection bias caused by difference in phage growth rate or even worseinstance ofusing pIII as the fusion partner can lead to a decrease in phage infectivity leading to problems0.80text
the phage's inability to infect its hostinstance ofusing pIII as the fusion partner can lead to a decrease in phage infectivity leading to problems0.80text
Phage displayhas applicationApplications0.60section
Phage displayhas applicationDNA0.60section
Phage displayhas applicationPhage0.60section
Phage displayhas applicationAs0.60section

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