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Directed evolution

Directed evolution (DE) is a method used in protein engineering that mimics the process of natural selection to steer proteins or nucleic acids toward a user-defined goal. It consists of subjecting a gene to iterative rounds of mutagenesis (creating a library of variants), selection (expressing those variants and isolating members with the desired…

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History

Principles

Comparison to rational protein design

Applications

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Map overview Semantic statistics

Directed evolution

Nodes95
Edges94
Triples83
Avg. degree1.98
Density0.021053
Components1

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Directed evolution

Top relations

related to External links · 20
Directed evolution → Archived, Dan Tawfik Research GroupThe, Directed Evolution Archived, Directed EvolutionCodexis, Frances Arnold Research Group, GroupSeSaM-Biotech, GroupThe Chang Liu Research, GroupThe Darren Hart Research, GroupThe David Liu Research, GroupThe Douglas Clark Research, GroupThe Ned Budisa Research, GroupThe Paul Dalby Research, Inc, Reetz, Research, Research GroupThe Donald Hilvert, Research GroupThe Manfred Reetz, Ulrich Schwaneberg Research GroupThe, Wayback MachineProf, Wayback MachineThe Huimin Zhao
related to Generating variation · 12
Directed evolution → DE, Depending, DNA, Earth, Even, Finally, Gene, Neither, Of, PCR, Some, The
related to history · 12
Directed evolution → Chemistry, Directed, Early, Frances Arnold, George Smith, Gregory Winter, Methods, Nobel Prize, RNA, Spiegelman's Monster, The, This
related to Adaptive laboratory evolution of microbial proteomes · 8
Directed evolution → Bacillus, Budisa, Directed, Escherichia, For, Söll, The, UGG
related to Combinatorial approaches · 7
Directed evolution → Alternatively, Beneficial, Combined, Creating, DE, Focused, For
related to Evolution studies · 7
Directed evolution → Additionally, DE, Directed, DNA, However, The, This
related to Limitations of directed evolution · 7
Directed evolution → Additionally, Consequently, DE, Improvement, There, This, To
related to Principles · 5
Directed evolution → Directed, Evolution, In DE, Rounds, The
is a · 1
Directed evolution → generation of a library of variant genes
has application · 1
Directed evolution → Directed

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Important terminology

evolution protein selection directed activity gene variants de mutagenesis used mutations screening library proteins cells genes engineering natural desired function

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Directed evolutionis ageneration of a library of variant genes0.90text
temperatureinstance ofboth the abiotic environment0.80text
and the biotic environmentinstance ofboth the abiotic environment0.80text
such as other genes in the organisminstance ofboth the abiotic environment0.80text
Directed evolutionhas applicationDirected0.60section
Directed evolutionrelated to Adaptive laboratory evolution of microbial proteomesThe0.60section
Directed evolutionrelated to Adaptive laboratory evolution of microbial proteomesFor0.60section
Directed evolutionrelated to Adaptive laboratory evolution of microbial proteomesEscherichia0.60section
Directed evolutionrelated to Adaptive laboratory evolution of microbial proteomesBacillus0.60section
Directed evolutionrelated to Adaptive laboratory evolution of microbial proteomesUGG0.60section
Directed evolutionrelated to Adaptive laboratory evolution of microbial proteomesBudisa0.60section
Directed evolutionrelated to Adaptive laboratory evolution of microbial proteomesSöll0.60section

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