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Confocal microscopy

Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. Capturing multiple two-dimensional images at different depths in a sample enables the reconstruction of three-dimensional structures (a process known as…

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MeSH
D018613
OPS-301 code
3-301

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Overview

Basic concept

Techniques used for horizontal scanning

Resolution enhancement

Uses

Variants and enhancements

Images

History

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Confocal microscopy

Nodes135
Edges134
Triples55
Avg. degree1.99
Density0.014815
Components1

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Confocal microscopy

Top relations

related to Resolution enhancement · 18
Confocal microscopy → AFM, Airy, CLSM, Confocal, For, Here, However, In, In CLSM, Increasing, It, One, SEM, STM, Such, The, This, When
related to Biology and medicine · 8
Confocal microscopy → Clinically, CLSM, Confocal, In, It, Research, Some, The
related to Low-temperature operability · 4
Confocal microscopy → Another, One, This, To
related to Material's surface characterization · 3
Confocal microscopy → During, Laser, Silicon
see also · 3
Confocal microscopy → Charge, TIRF, Two-photon
related to Audio preservation · 2
Confocal microscopy → The IRENE, Wax
MeSH · 1
Confocal microscopy → D018613
OPS-301 code · 1
Confocal microscopy → 3-301
is a · 1
Confocal microscopy → optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation

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Important terminology

confocal microscope scanning laser resolution microscopy light pinhole used optical sample first point imaging microscopes fluorescence clsm using image illumination

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Confocal microscopyMeSHD0186131.00infobox
Confocal microscopyOPS-301 code3-3011.00infobox
Confocal microscopyis aoptical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation0.90text
live cell imaging.Cutting-edge development of confocal laser scanning microscopy now allows better than standard video rateinstance ofa desirable feature for dynamic observations0.80text
perfluorodecalininstance ofnon-toxic perfluorocarbons0.80text
which readily infiltrates tissuesinstance ofnon-toxic perfluorocarbons0.80text
has a refractive index almost identical to that of waterinstance ofnon-toxic perfluorocarbons0.80text
stimulated emission depletion microscopyinstance ofimproving contrast in both the axial and lateral planes.Super resolutionThere are confocal variants that achieve resolution below the diffraction limit0.80text
ease of useinstance ofThey all have their own advantages0.80text
resolutioninstance ofThey all have their own advantages0.80text
and the need for special equipmentinstance ofThey all have their own advantages0.80text
buffersinstance ofThey all have their own advantages0.80text

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