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A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution. With it the typical range of the axial resolution of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy.
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light microscope 4pi resolution microscopy objective emission lenses excitation axial two superposition possible solid angle displaystyle focal improved omega pi
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| 4Pi microscope | is a | laser scanning fluorescence microscope with an improved axial resolution | 0.90 | text |
| 4Pi microscope | related to history | In | 0.60 | section |
| 4Pi microscope | related to history | Christoph Cremer | 0.60 | section |
| 4Pi microscope | related to history | Thomas Cremer | 0.60 | section |
| 4Pi microscope | related to history | However | 0.60 | section |
| 4Pi microscope | related to history | The | 0.60 | section |
| 4Pi microscope | related to history | Stefan Hell | 0.60 | section |
| 4Pi microscope | related to history | He | 0.60 | section |
| 4Pi microscope | related to history | For | 0.60 | section |
| 4Pi microscope | related to history | Leica Microsystems | 0.60 | section |
| 4Pi microscope | related to Working principle | The | 0.60 | section |
| 4Pi microscope | related to Working principle | Also | 0.60 | section |
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