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Reverse phase protein lysate microarray

A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large number of biological samples simultaneously in a quantitative manner when high-quality antibodies are available.

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Experimental design and procedure

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Reverse phase protein lysate microarray

Nodes32
Edges31
Triples24
Avg. degree1.94
Density0.0625
Components1

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Important terminology

protein rpma antibody antibodies microarray samples used lysates detection signal cell capture using proteins phase forward sample rpmas different arrays

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
seruminstance ofbody fluids0.80text
CSFinstance ofbody fluids0.80text
urineinstance ofbody fluids0.80text
vitreousinstance ofbody fluids0.80text
salivainstance ofbody fluids0.80text
etc.instance ofbody fluids0.80text
are immobilized on individual spots on a microarray that is then incubated with a single specific antibody to detect expression of the target protein across many samplesinstance ofbody fluids0.80text
ELISAinstance ofas is seen in other techniques0.80text
or immunohistochemistryinstance ofas is seen in other techniques0.80text
the signal from a single spot could be due to cross-reactivityinstance ofas is seen in other techniques0.80text
quantitative analysis of protein expression in cancer cellsinstance ofthe antibodies used in RPMA must be carefully validated for specificity and performance against cell lysates by western blot.RPMA has various uses0.80text
body fluids or tissues for biomarker profilinginstance ofthe antibodies used in RPMA must be carefully validated for specificity and performance against cell lysates by western blot.RPMA has various uses0.80text

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