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Lysis buffer

A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction). Most lysis buffers contain buffering salts (e.g. Tris-HCl) and ionic salts (e.g. NaCl) to regulate the pH and osmolarity of the…

Applications, Overview & Commonly used buffers

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Overview

Choosing a buffer

Commonly used buffers

Detergents, salts & enzymes

Advanced semantic analysis

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Map overview Semantic statistics

Lysis buffer

Nodes34
Edges33
Triples27
Avg. degree1.94
Density0.058824
Components1

How this topic connects Entity context

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Lysis buffer

Top relations

related to Choosing a buffer · 7
Lysis buffer → Different, For, Gram-negative, Gram-positive, It, The, Thus
related to Lysis buffer in DNA and RNA studies · 6
Lysis buffer → Buffer P2, Dish, DNA, In, Other, Qiagen
related to NP-40 lysis buffer · 6
Lysis buffer → It, NP-40, Recipe, RIPA, Since NP-40, The
related to GentleLys (Gentle Lysis) · 3
Lysis buffer → Consequently, The GentleLys, This
related to RIPA (RadioImmunoPrecipitation Assay) lysis buffer · 3
Lysis buffer → Recipe, RIPA, The
is a · 1
Lysis buffer → buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells

Important terminology Word statistics

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Important terminology

lysis buffer used buffers detergents cell protein proteins salts detergent lysing ph sds components ionic cells commonly nacl target include

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Lysis bufferis abuffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells0.90text
white blood cells are of greater interest.Recipeinstance oflysing bufferACK is used for lysis of red blood cells in biological samples where other cells0.80text
Lysis bufferrelated to Choosing a bufferThe0.60section
Lysis bufferrelated to Choosing a bufferFor0.60section
Lysis bufferrelated to Choosing a bufferDifferent0.60section
Lysis bufferrelated to Choosing a bufferThus0.60section
Lysis bufferrelated to Choosing a bufferGram-negative0.60section
Lysis bufferrelated to Choosing a bufferGram-positive0.60section
Lysis bufferrelated to Choosing a bufferIt0.60section
Lysis bufferrelated to GentleLys (Gentle Lysis)The GentleLys0.60section
Lysis bufferrelated to GentleLys (Gentle Lysis)This0.60section
Lysis bufferrelated to GentleLys (Gentle Lysis)Consequently0.60section

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