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Immunostaining

In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. However, immunostaining now encompasses a broad range of techniques used in histology…

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Techniques

TissueImmunocytochemistryCellsFluorescentDyes
31 related topics

Methodological overview

EpitopeMonoclonalPolyclonalFluorophoreBiotin
9 related topics

Applications

Histopathology
1 related topics

Overview

BiochemistryAntibodyProteinImmunohistochemical stainingAlbert Coons
8 related topics

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Immunostaining

Nodes54
Edges53
Triples21
Avg. degree1.96
Density0.037037
Components1

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Immunostaining

Top relations

related to Immunohistochemistry · 11
Immunostaining → Alternatively, However, IHC, Immunohistochemistry, Inappropriate, Many, The, These, Tissue, Under, While
related to overview · 4
Immunostaining → Detection, In, The, These
has application · 3
Immunostaining → Clinically, IHC, The
see also · 1
Immunostaining → Cutaneous

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Important terminology

used tissue protein methods using antibody specific staining microscopy fixation citation needed sections antibodies cell cells ihc detection flow blotting

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
peroxidaseinstance ofother non-fluorescent methods using enzymes0.80text
horseradish peroxidase or alkaline phosphatase are commonly used to catalyse reactions that give a coloured or chemiluminescent productinstance ofenzymes0.80text
Immunostaininghas applicationThe0.60section
Immunostaininghas applicationClinically0.60section
Immunostaininghas applicationIHC0.60section
Immunostainingrelated to ImmunohistochemistryImmunohistochemistry0.60section
Immunostainingrelated to ImmunohistochemistryIHC0.60section
Immunostainingrelated to ImmunohistochemistryWhile0.60section
Immunostainingrelated to ImmunohistochemistryThese0.60section
Immunostainingrelated to ImmunohistochemistryAlternatively0.60section
Immunostainingrelated to ImmunohistochemistryTissue0.60section
Immunostainingrelated to ImmunohistochemistryInappropriate0.60section

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