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Immunofluorescence

Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens. The specific region an antibody recognizes on an antigen is called an epitope. Several…

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Immunofluorescence

Nodes37
Edges36
Triples53
Avg. degree1.95
Density0.054054
Components1

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Immunofluorescence

Top relations

related to Advances · 9
Immunofluorescence → Fluorophores, FPALM, Some, SSIM, STED, STORM, Super-resolution, The, This
related to External links · 9
Immunofluorescence → Archived, BirminghamOverview, Davidson CollegeImmunofluorescence, Images, Medicine Medical Subject Headings, MeSH, National Library, University, Wayback Machine
related to Primary (direct) · 9
Immunofluorescence → DIF, IF, Indirect, One, Primary, Secondary, The, This, When
related to Limitations · 8
Immunofluorescence → Alexa Fluors, By, DyLight Fluors, GFP, One, Seta Fluors, The GFP-technique, To
related to Preparation of fluorescence · 4
Immunofluorescence → Staining, The, There, To
related to Secondary (indirect) · 3
Immunofluorescence → Secondary, SIF, The
see also · 2
Immunofluorescence → AntibodiesCutaneous, Capping
is a · 1
Immunofluorescence → widely used example of immunostaining

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Important terminology

antibodies fluorophore antibody epitope fluorescence cell technique specific used proteins antigen light secondary fluorescent staining target within structures primary conjugated

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Immunofluorescenceis awidely used example of immunostaining0.90text
intermediate-sized filaments.If the topology of a cell membrane is undeterminedinstance ofand visualization of structures0.80text
epitope insertion into proteins can be used in conjunction with immunofluorescence to determine structures within the cell membraneinstance ofand visualization of structures0.80text
the Avidin-Biotin Complexinstance ofThere are different methods for attaining a higher fluorophore-antigen ratio0.80text
Alexa Fluorsinstance ofor opt for more robust fluorophores that exhibit resilience against photobleaching0.80text
Seta Fluorsinstance ofor opt for more robust fluorophores that exhibit resilience against photobleaching0.80text
or DyLight Fluors.Other problems that may arise when using immunofluorescence techniques include autofluorescenceinstance ofor opt for more robust fluorophores that exhibit resilience against photobleaching0.80text
spectral overlapinstance ofor opt for more robust fluorophores that exhibit resilience against photobleaching0.80text
non-specific staininginstance ofor opt for more robust fluorophores that exhibit resilience against photobleaching0.80text
Immunofluorescencerelated to AdvancesThe0.60section
Immunofluorescencerelated to AdvancesFluorophores0.60section
Immunofluorescencerelated to AdvancesSuper-resolution0.60section

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