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Heteronuclear single quantum coherence spectroscopy

The heteronuclear single quantum coherence or heteronuclear single quantum correlation experiment, normally abbreviated as HSQC, is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR. The experiment was first described by Geoffrey Bodenhausen and D. J. Ruben in 1980. The resulting…

Art, HSQC in protein NMR & General scheme

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HSQC in protein NMR

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Heteronuclear single quantum coherence spectroscopy

Nodes33
Edges32
Triples7
Avg. degree1.94
Density0.060606
Components1

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hsqc protein nmr experiment 15n spectrum 1h peaks normally proton 13c also peak attached experiments used usually 31p residue structure

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SubjectPredicateObjectConfidenceSrc
1Hinstance ofbut is also applicable to other nuclei0.80text
structure determinationinstance ofThe assignment of the spectrum is essential for a meaningful interpretation of more advanced NMR experiments0.80text
relaxation analysis.Chemicals labelled with 15N isotope are relatively inexpensiveinstance ofThe assignment of the spectrum is essential for a meaningful interpretation of more advanced NMR experiments0.80text
and the 15N HSQC is a sensitive experiment whereby a spectrum can be acquired in a relatively short timeinstance ofThe assignment of the spectrum is essential for a meaningful interpretation of more advanced NMR experiments0.80text
the 15N HSQC is therefore often used to screen candidates for their suitability for structure determination by NMRinstance ofThe assignment of the spectrum is essential for a meaningful interpretation of more advanced NMR experiments0.80text
as well as optimization of the sample conditionsinstance ofThe assignment of the spectrum is essential for a meaningful interpretation of more advanced NMR experiments0.80text
drugsinstance ofas well the interactions with ligands0.80text

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