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ELISpot

The enzyme-linked immunosorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell. The ELISpot Assay is also a form of immunostaining since it is classified as a technique that uses antibodies to detect a protein analyte, with the word analyte referring to any biological or…

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Mechanism of ELISpot

Monoclonal antibodiesEpitopePolyclonalPhosphate buffered salineGranulocytes
10 related topics

History

Hybridoma cellsELISALymphokineT cells
4 related topics

Applications of ELISpot and FluoroSpot

Granzyme BImmunoglobulins
2 related topics

Overview

AssayCytokineImmunostainingAntibodiesAnalyte
6 related topics

Topics to explore

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Overview

History

Mechanism of ELISpot

Applications of ELISpot and FluoroSpot

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Map overview Semantic statistics

ELISpot

Nodes27
Edges26
Triples78
Avg. degree1.93
Density0.074074
Components1

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ELISpot

Top relations

related to Mechanism of ELISpot · 31
ELISpot → Addition, All, ALP, Analysis, Antibody, Any, At, BCIP/NBT-plus, Biotinylated, Biotinylation, Cell, Celsius, Cytokine, Detection, During, Each, ELISpot Assay, HRP, If, PBS
related to Mechanism of FluoroSpot · 25
ELISpot → After, Allow, Analysis, Antibody Coating, Cell, Cell Incubation, Cytokine Capture, Detection Antibodies, Ethanol, Fluorophore-labeled Conjugates, FluoroSpot, For, For MAIPSWU, For MSIP, In, Instead, IPFL, Once, Proteins/analytes, Similar
has application · 18
ELISpot → FluoroSpot, IFN-y, IL-13, IL-2, IL-4, IL-5, Measuring T-cell, More, Perforin, T-cell, T-cell ELISpot, Th1, Th2, The, The ELISpot, This, TNF-alpha, With
related to history · 4
ELISpot → Cecil Czerkinsky, Czerkinsky, ELISA, In

Important terminology Word statistics

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Important terminology

wells assay fluorospot antibodies cytokine cells added used secretion ethanol fluorescence also antibody detection order multiple analytes analyte first capture

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
ELISpothas applicationThe ELISpot0.60section
ELISpothas applicationFluoroSpot0.60section
ELISpothas applicationWith0.60section
ELISpothas applicationPerforin0.60section
ELISpothas applicationT-cell0.60section
ELISpothas applicationIL-40.60section
ELISpothas applicationIL-50.60section
ELISpothas applicationIL-130.60section
ELISpothas applicationMore0.60section
ELISpothas applicationT-cell ELISpot0.60section
ELISpothas applicationThis0.60section
ELISpothas applicationIFN-y0.60section

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