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A DNA construct is an artificially-designed segment of DNA borne on a vector that can be used to incorporate genetic material into a target tissue or cell. A DNA construct contains a DNA insert, called a transgene, delivered via a transformation vector which allows the insert sequence to be replicated and/or expressed in the target cell. This gene can be…
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dna vector gene constructs target using sequence used vectors construct expression cell sequences plasmids cloning protein restriction naturally methods typically
| Subject | Predicate | Object | Confidence | Src |
|---|---|---|---|---|
| DNA construct | is a | artificially-designed segment of DNA borne on a vector that can be used to incorporate genetic material into a target tissue or cell | 0.90 | text |
| plasmids or bacteriophages | instance of | using vectors | 0.80 | text |
| or as large as hundreds of kbp for large-scale genomic studies using an artificial chromosome | instance of | using vectors | 0.80 | text |
| DNA sequencing | instance of | DNA constructs are widely adapted in molecular biology research for techniques | 0.80 | text |
| protein expression | instance of | DNA constructs are widely adapted in molecular biology research for techniques | 0.80 | text |
| and RNA studies | instance of | DNA constructs are widely adapted in molecular biology research for techniques | 0.80 | text |
| Lac inhibitors | instance of | regulatory elements | 0.80 | text |
| a polylinker | instance of | regulatory elements | 0.80 | text |
| and a protein tag which facilitates protein purification.Bacteriophage Vectors are viruses that can infect bacteria | instance of | regulatory elements | 0.80 | text |
| replicate their own DNA.Artificial chromosomes are commonly used in genome project studies due to their ability to hold inserts up to 350 kbp | instance of | regulatory elements | 0.80 | text |
| promoters | instance of | DNA constructs can also change the expression levels of other genes by expressing regulatory sequences | 0.80 | text |
| inhibitors | instance of | DNA constructs can also change the expression levels of other genes by expressing regulatory sequences | 0.80 | text |
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