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Slice preparation

The slice preparation or brain slice is a laboratory technique in electrophysiology that allows the study of neurons from various brain regions in isolation from the rest of the brain, in an ex-vivo condition. Brain tissue is initially sliced via a tissue slicer then immersed in artificial cerebrospinal fluid (aCSF) for stimulation and/or recording. The…

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Overview

Slice preparation techniques

Benefits

Limitations

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Slice preparation

Nodes29
Edges28
Triples27
Avg. degree1.93
Density0.068966
Components1

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Slice preparation

Top relations

related to Limitations · 9
Slice preparation → Additionally, Compresstome, During, Finally, Further, Most, Slice, The, To
related to Benefits · 7
Slice preparation → CNS, Finally, Furthermore, Removing, Slice, The, When
related to Bibliography · 6
Slice preparation → Avital, Brain Slice Preparation, Electrophysiology, Kopf Carrier, Schurr, Vol

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Important terminology

slice brain preparation tissue allows also recording fluid slicing may effects technique rest control electrophysiology circuit solution sample used vivo

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
sample size limitationinstance ofThis method has several drawbacks0.80text
difficult to observe progressinstance ofThis method has several drawbacks0.80text
Compresstome microtomes are used to prepare slices as these devices have less limitationsinstance ofModern microtome devices0.80text
the Compresstomeinstance ofa more sophisticated tissue slicer may be used0.80text
a type of vibrating microtome used to maximizes the amount of viable tissue cellsinstance ofa more sophisticated tissue slicer may be used0.80text
Slice preparationrelated to BenefitsWhen0.60section
Slice preparationrelated to BenefitsCNS0.60section
Slice preparationrelated to BenefitsSlice0.60section
Slice preparationrelated to BenefitsThe0.60section
Slice preparationrelated to BenefitsRemoving0.60section
Slice preparationrelated to BenefitsFurthermore0.60section
Slice preparationrelated to BenefitsFinally0.60section

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