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Multilocus sequence typing

Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci, using DNA sequences of internal fragments of multiple housekeeping genes to characterize isolates of different species. It is most commonly applied to microbes, mostly prokaryotes and yeasts, as they tend to be hard to differentiate using other means.

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Principle

PCRDNA sequencingSequence analysisAlleleStaphylococcus aureus
25 related topics

Advantages and applications

BioinformaticsCampylobacterMRSAS. pyogenesC. albicans
7 related topics

Comparison with other techniques

PFGEPCR-based fingerprintingMulti locus enzyme electrophoresisDNA barcoding
4 related topics

Databases

Wayback Machine
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Multilocus sequence typing

Nodes51
Edges50
Triples80
Avg. degree1.96
Density0.039216
Components1

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Multilocus sequence typing

Top relations

related to Further reading · 72
Multilocus sequence typing → Acad, Arduino, Arduino SM, Bibcode, Bygraves JA, Chen, Clin, Comparison, CS1, Differentiating Salmonella, Discriminatory Ability, Duh, Duh RW, Epidemiology, Fakhr MK, Feil, Foley SL, Food Animal Sources, Harris, Harris AD

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Important terminology

mlst sequence isolates typing sequences genes used dna housekeeping different 10 species loci multiple doi pmid data analysis also using

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
reliance on few antigenic lociinstance ofbut immunological typing has drawbacks0.80text
unpredictable reactivities of antibodies with different antigenic variantsinstance ofbut immunological typing has drawbacks0.80text
pulsed-field gel electrophoresisinstance ofSeveral molecular typing schemes have been proposed to determine the relatedness of pathogens0.80text
outbreakinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
hostinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
geographical locationinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
etc.instance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
and allows easy comparison to typing results obtained using other typing methodsinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
Multilocus sequence typingrelated to Further readingMaiden MC0.60section
Multilocus sequence typingrelated to Further readingBygraves JA0.60section
Multilocus sequence typingrelated to Further readingFeil0.60section
Multilocus sequence typingrelated to Further readingMarch0.60section

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