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Multilocus sequence typing: Applications, Measurement & Standards

Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci, using DNA sequences of internal fragments of multiple housekeeping genes to characterize isolates of different species. It is most commonly applied to microbes, mostly prokaryotes and yeasts, as they tend to be hard to differentiate using other means.

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Multilocus sequence typing topic overview

The analysis highlights Applications, Measurement and Standards as prominent areas in the source structure around Multilocus sequence typing.

Related topics
43
Source areas
5
Connected nodes
48
Extracted relationships
8
Related term clusters
11
Bridge connections
48

What this topic covers Research coverage

Source areas are shown by the number of related topics found in each part of the analysis. Use smaller areas too: they can reveal specialized angles and content gaps.

Principle · 25 topics
Overview · 8 topics
Advantages and applications · 7 topics
Comparison with other techniques · 2 topics
Databases · 1 topics

Smaller areas are not necessarily less important. They contain fewer connections in this analysis and can be useful for finding specialized angles or coverage gaps.

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Explore all related topics Closing gaps

Browse the complete topic structure, not only the most central items. Less prominent entities and concepts can reveal missing angles, specialized context and useful research gaps. Each item opens a new analysis centered on that subject.

Overview

Principle

Comparison with other techniques

Advantages and applications

Databases

For the semantics nerds

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Advanced semantic analysis

How Multilocus sequence typing connects Entity context

See recurring relationship patterns around Multilocus sequence typing before inspecting the individual extracted relationships.

Important terminology

Use these terms to understand the vocabulary surrounding the topic, not as a checklist for keyword stuffing.

Important terminology

mlst sequence isolates typing sequences genes used dna housekeeping different 10 species loci multiple doi pmid data analysis also using

Multilocus sequence typing relationships Subject–Predicate–Object triples

TTTA extracted 8 structured relationships around Multilocus sequence typing. Examples in this analysis include reliance on few antigenic loci → instance of → but immunological typing has drawbacks and pulsed-field gel electrophoresis → instance of → Several molecular typing schemes have been proposed to determine the relatedness of pathogens. The table shows each extracted connection, where it came from and its confidence.

SubjectPredicateObjectConfidenceSrc
reliance on few antigenic lociinstance ofbut immunological typing has drawbacks0.80text
unpredictable reactivities of antibodies with different antigenic variantsinstance ofbut immunological typing has drawbacks0.80text
pulsed-field gel electrophoresisinstance ofSeveral molecular typing schemes have been proposed to determine the relatedness of pathogens0.80text
outbreakinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
hostinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
geographical locationinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
etc.instance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text
and allows easy comparison to typing results obtained using other typing methodsinstance ofAllele sequences and IDs can also be derived from whole genome sequences.CLC Microbial Genomics Module includes a set of tools and ready-to-use workflows for MLST in the context…0.80text

Related concept clusters Related term clusters

The concept neighborhoods around Multilocus sequence typing bring nearby vocabulary together. In this analysis, examples include Sequence, Typing and Multiple. Use the clusters to find adjacent concepts and terminology that may deserve separate research.

  • Multilocus sequence typing
    • Sequence
    • Typing
    • Multiple
    • Link
    • Pmid
    • Using
    • Analysis
    • Strains
    • Isolates
    • Allele
    • Cronobacter
    • Bacterial
  • multilocus sequence typing
    • Sequence
    • Typing
    • Multiple
    • Link
    • Using
    • Analysis
    • Data
    • Pmid
    • Identification
    • Sequences
    • Strains
    • Isolates
  • loci
    • Different
    • Isolates
    • Alleles
    • Allelic
    • Used
    • Housekeeping
    • Discriminatory
    • Power
    • Technique
    • Bacterial
    • Method
    • Profiles
  • dna sequences
    • Allele
    • Technique
    • Alleles
    • Sequence
    • Housekeeping
    • Analysis
    • Use
    • Genes
    • Strains
    • Species
    • Mlst
    • Different
  • housekeeping genes
    • Genes
    • Housekeeping
    • Strains
    • Alleles
    • Allelic
    • Loci
    • Different
    • Power
    • Used
    • Mlst
    • Discriminatory
    • Use
  • dna sequencing
    • Technique
    • Bacterial
    • Sequence
    • Housekeeping
    • Use
    • Genes
    • Strains
    • Species
    • Mlst
    • Different
    • Identification
    • Sequences
  • sequence analysis
    • Typing
    • Analysis
    • Data
    • Sequence
    • Allele
    • Multilocus
    • Sequences
    • Strains
    • Multiple
    • Link
    • Cronobacter
    • Discriminatory
  • dna barcoding
    • Technique
    • Sequence
    • Housekeeping
    • Use
    • Genes
    • Strains
    • Species
    • Mlst
    • Different
    • Sequences
    • Campylobacter
    • Cronobacter

Connections between topic areas Semantic bridges

For Multilocus sequence typing, one of the stronger structural bridges in this analysis connects Multilocus sequence typing with Principle. Bridges highlight paths between different parts of the map and can reveal research angles that are easy to miss in a flat list.

Min side: 3
Multilocus sequence typing — Principle · splits 23 ⟂ 26
Multilocus sequence typing — Overview · splits 40 ⟂ 9
Multilocus sequence typing — Advantages and applications · splits 41 ⟂ 8
Multilocus sequence typing — Comparison with other techniques · splits 46 ⟂ 3

Map overview Semantic statistics

Multilocus sequence typing

Nodes49
Edges48
Triples8
Avg. degree1.96
Density0.040816
Components1

Source & methodology

TTTA analyzes the structure around Multilocus sequence typing to surface related topics, entities, relationships, concept neighborhoods and bridge connections. Use the map to explore areas such as Applications, Measurement & Standards, including less central topics that may reveal useful research gaps. Automatically extracted connections are research leads rather than rewritten encyclopedia content.

Source: Wikipedia — Multilocus sequence typing · EN edition · Analysis: TopicsToTalkAbout

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