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Linear epitope

In immunology, a linear epitope (also sequential epitope) is an epitope—a binding site on an antigen—that is recognized by antibodies by its linear sequence of amino acids (i.e. primary structure). In contrast, most antibodies recognize a conformational epitope that has a specific three-dimensional shape (tertiary structure).

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Significance

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Linear epitope

Nodes25
Edges24
Triples16
Avg. degree1.92
Density0.08
Components1

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Linear epitope

Top relations

related to Significance · 7
Linear epitope → ELISA, For, In, SDS-PAGE, Therefore, Western, While

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Important terminology

antigen linear amino acids called epitope proteins antibodies recognize epitopes structure conformational protein sequential recognized sequence primary specific segments antibody

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
in the Western blotinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
immunohistochemistryinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
and ELISAinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
one should carefully choose antibodies that recognize linear or conformational epitopes.For instanceinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
if a protein sample is boiledinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
treated with beta-mercaptoethanolinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
and run in SDS-PAGE for the Western blotinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
the proteins are essentially denaturedinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
therefore cannot assume their natural three-dimensional conformationsinstance ofSignificanceWhile performing molecular assays involving use of antibodies0.80text
Linear epitoperelated to SignificanceWhile0.60section
Linear epitoperelated to SignificanceWestern0.60section
Linear epitoperelated to SignificanceELISA0.60section

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