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High-performance liquid chromatography

High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a chromatography technique in analytical chemistry used to separate, identify, and quantify specific components (analytes) in mixtures. The mixtures can originate from food, chemicals, pharmaceuticals, biological, environmental and agriculture…

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Operation

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Types

Isocratic and gradient elution

Parameters

Applications

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High-performance liquid chromatography

Nodes145
Edges144
Triples30
Avg. degree1.99
Density0.013793
Components1

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Important terminology

phase hplc column chromatography mobile retention used stationary separation sample analytes time elution also gradient surface liquid analyte silica water

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
UV detectorsinstance ofThe eluate enters a specific chromatography detector0.80text
which produces a graphinstance ofThe eluate enters a specific chromatography detector0.80text
silicainstance ofthis method separates analytes based on their affinity for a polar stationary surface0.80text
traces of water in the mobile phase tend to adsorb to the solid surface of the stationary phase forming a stationary boundinstance ofVery polar solvents0.80text
ODSinstance ofsometimes called by a trade names0.80text
C18H37 or C8H17.To separate aromatic compoundsinstance ofwhere R is a straight chain alkyl group0.80text
or in general compounds with many pi bondsinstance ofwhere R is a straight chain alkyl group0.80text
the stationary phase can have phenyl surface ligands.At pHinstance ofwhere R is a straight chain alkyl group0.80text
acetic acid or formic acidinstance ofthen a volatile organic acid often added to the eluent0.80text
carboxylic acidsinstance ofTFA also increases retention of analytes0.80text
in applications utilizing other detectorsinstance ofTFA also increases retention of analytes0.80text
proteins or polymersinstance ofSEC is used primarily for the analysis of large molecules0.80text

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