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Isoelectric focusing

Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different charged molecules by differences in their isoelectric point (pI). It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest, i.e. the net charge density, is a…

Standards, Procedure & Living cells

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Procedure

Ampholyte Immobilized pH gradient Acrylamide Aliphatic Polyampholytes

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Overview

Charged Electric charge
Molecules
Isoelectric point
Electrophoresis
Proteins Protein
Gel Gel electrophoresis
Charge density
PH

Procedure

Living cells

Microfluidic chip based

Capillary electrophoresis

Multi-junction

Alzheimer's disease

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Isoelectric focusing

Nodes35

Edges34

Triples7

Avg. degree1.94

Density0.057143

Components1

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Isoelectric focusing

Top relations

related to Living cells · 2

Isoelectric focusing → According, By

is a · 1

Isoelectric focusing → first step in two-dimensional gel electrophoresis

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Important terminology

ph proteins pi gradient isoelectric charge point molecules ief gel capillary focusing electrophoresis protein multi-junction charged value toward different usually

Entity relationships Subject–Predicate–Object triples

Subject	Predicate	Object	Confidence	Src
Isoelectric focusing	is a	first step in two-dimensional gel electrophoresis	0.90	text
polyampholytes with varying pI values to electrophoresis.The method is applied particularly often in the study of proteins	instance of	The gradient is established before adding the particles of interest by first subjecting a solution of small molecules	0.80	text
which separate based on their relative content of acidic	instance of	The gradient is established before adding the particles of interest by first subjecting a solution of small molecules	0.80	text
basic residues	instance of	The gradient is established before adding the particles of interest by first subjecting a solution of small molecules	0.80	text
whose value is represented by the pI	instance of	The gradient is established before adding the particles of interest by first subjecting a solution of small molecules	0.80	text
Isoelectric focusing	related to Living cells	According	0.60	section
Isoelectric focusing	related to Living cells	By	0.60	section

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