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An autonomously replicating sequence (ARS or ars) contains the origin of replication in the yeast genome. The ARS of S. cerevisiae is a minimal 125 bp, and contains four regions (A, B1, B2, and B3), named in order of their effect on plasmid stability. The A-Domain is highly conserved, any mutation abolishes origin function. Mutations on B1, B2, and B3…
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ars origin b1 b2 b3 yeast genome mutation function element histidine autonomously replicating sequence contains replication regions highly conserved replicative
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