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Single-molecule real-time sequencing

Single-molecule real-time (SMRT) sequencing is a parallelized single molecule DNA sequencing method. Single-molecule real-time sequencing utilizes a zero-mode waveguide (ZMW). A single DNA polymerase enzyme is affixed at the bottom of a ZMW with a single molecule of DNA as a template. The ZMW is a structure that creates an illuminated observation volume…

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Application

DideoxynucleotideBroad InstituteMethylatedMedical genetics
4 related topics

History

ReagentAmplicon
2 related topics

Technology

DNA synthesisPhosphodiester bondNanophotonic
3 related topics

Sequencing Performance

IlluminaPacific Biosciences
2 related topics

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Technology

Sequencing Performance

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Application

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Single-molecule real-time sequencing

Nodes23
Edges22
Triples9
Avg. degree1.91
Density0.086957
Components1

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Single-molecule real-time sequencing

Top relations

related to Application · 8
Single-molecule real-time sequencing → Celera Assembler, For, In, Sanger, Scientists, Single-molecule, SMRT, The

Important terminology Word statistics

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Important terminology

sequencing dna bases read smrt zmw length polymerase chemistry nucleotide released throughput 000 cell fluorescent per average increased molecule lengths

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
BluePippininstance ofusing an electrophoresis instrument0.80text
Single-molecule real-time sequencingrelated to ApplicationSingle-molecule0.60section
Single-molecule real-time sequencingrelated to ApplicationFor0.60section
Single-molecule real-time sequencingrelated to ApplicationSanger0.60section
Single-molecule real-time sequencingrelated to ApplicationThe0.60section
Single-molecule real-time sequencingrelated to ApplicationScientists0.60section
Single-molecule real-time sequencingrelated to ApplicationIn0.60section
Single-molecule real-time sequencingrelated to ApplicationCelera Assembler0.60section
Single-molecule real-time sequencingrelated to ApplicationSMRT0.60section

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