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Immunogold labelling

Immunogold labeling or immunogold staining (IGS) is a staining technique used in electron microscopy. This staining technique is an equivalent of the indirect immunofluorescence technique for visible light. Colloidal gold particles are most often attached to secondary antibodies which are in turn attached to primary antibodies designed to bind a specific…

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SalmonellaAntigensProteins
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Technique

MicrotomeSample preparationProtein AProtein GIgG
6 related topics

Uses in brightfield microscopy

AcidicSolutionIonsNucleationPathogen
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Limitations

NmThree-dimensional structureMicrotubuleThree-dimensionalResin
5 related topics

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Technique

Labeling multiple objects

Uses in brightfield microscopy

Limitations

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Immunogold labelling

Nodes40
Edges39
Triples1
Avg. degree1.95
Density0.05
Components1

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gold labeling immunogold particles electron used microscopy technique using sample limitation attached secondary brightfield antibodies cell antibody primary bind first

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SubjectPredicateObjectConfidenceSrc
immunogoldinstance ofso did the need for nanoparticle-sized labels0.80text

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