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Sanger sequencing

Sanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being developed by Frederick Sanger and colleagues in 1977, it became the most widely used sequencing method for approximately 40…

Companies, Method & Microfluidic Sanger sequencing

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Overview

Method

Microfluidic Sanger sequencing

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Map overview Semantic statistics

Sanger sequencing

Nodes64
Edges63
Triples69
Avg. degree1.97
Density0.03125
Components1

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Sanger sequencing

Top relations

has application · 22
Sanger sequencing → BioNumerics, CalciNet, CaliciNet, CDC, Center, COVID-19, Disease Control, January, Laboratories, Many, March, PHL, Prevention's, Public, S-gene, Sanger, SARS-CoV-2, Sequences, The, The CalciNet
related to Challenges · 10
Sanger sequencing → Ampliseq, Base, Common, DNA, In, PCR-based, Phred, Recently, Sanger, SeqSharp
related to Microfluidic Sanger sequencing · 10
Sanger sequencing → Adopting, Amplified, CAE, DNA, In, Microfluidic Sanger, PCR, Sanger, These, This
related to Device design · 9
Sanger sequencing → After, CE, PDMS, Reaction, Sanger, TC, The, The CE, Three-dimensional
related to Platforms · 6
Sanger sequencing → California, Dublin, Microchip Biotechnologies Inc, Sanger, The, The Apollo
is a · 2
Sanger sequencing → lab-on-a-chip application for DNA sequencing, method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA rep…

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Important terminology

sequencing dna sanger sequence electrophoresis method automated methods fragments capillary using genome data fluorescent nucleotides four reaction polymerase surveillance used

Entity relationships Subject–Predicate–Object triples

SubjectPredicateObjectConfidenceSrc
Sanger sequencingis amethod of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA rep…0.90text
Sanger sequencingis alab-on-a-chip application for DNA sequencing0.90text
sequencing the spike protein from SARS-CoV-2 as well as for the surveillance of norovirus outbreaks through the United States Center for Disease Controlinstance ofSanger sequencing is still actively being used in efforts for public health initiatives0.80text
Preventioninstance ofSanger sequencing is still actively being used in efforts for public health initiatives0.80text
the S-geneinstance ofothers have opted to sequence very specific genes of the virus0.80text
encoding the information needed to produce the spike proteininstance ofothers have opted to sequence very specific genes of the virus0.80text
next generation sequencinginstance ofLaboratories in lower income countries may not have the capabilities to implement expensive applications0.80text
so Sanger methods may prevail in supporting the generation of sequencing data for surveillance of variants.Sanger sequencing is also theinstance ofLaboratories in lower income countries may not have the capabilities to implement expensive applications0.80text
Phred typically provides an estimate of quality to aid in trimming of low-quality regions of sequences.In cases where DNA fragments are cloned before sequencinginstance ofBase calling software0.80text
the resulting sequence may contain parts of the cloning vectorinstance ofBase calling software0.80text
Ampliseqinstance ofmethods0.80text
SeqSharp have been developed that allow rapid sequencing of target genes without cloning or prior amplification.Current methods can directly sequence only relatively shortinstance ofmethods0.80text

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